Moscow
Scientists have developed and tested
a new type of artificial blood vessel coating that can resist blood clot
formation.
The achievement was made possible by
a new generation of drug-containing coating applied to the inner surface of the
vessel, researchers said.
Surgery, associated with
cardiovascular diseases, such as ischemia, often require the implantation of
vascular grafts — artificial blood vessels, aimed at restoring the blood flow
in a problematic part of the circulatory system.
A serious disadvantage of vascular
grafts is their tendency to get blocked due to clot formation, which results in
compulsory and lifelong intake of anticoagulants among patients and sometimes
may even require an additional surgical intervention.
In the study, a research team led by
Vladimir Vinogradov, head of the International Laboratory of Solution Chemistry
of Advanced Materials and Technologies at ITMO University in Saint Petersburg,
Russia, proposed a solution to the problem.
The team managed to synthesise a
thin film made of densely packed aluminium oxide nanorods blended with
molecules of a thrombolytic enzyme (urokinase-type plasminogen activator).
Adhered to the inner surface of a
vascular graft, the film causes the parietal area of the graft to get filled
with a stable concentration of a substance, called plasmin, which is capable of
dissolving the appearing clots.
The unique properties of the film
arise from its structure, which represents a porous matrix, accommodating the
plasminogen activator.
The matrix protects the plasminogen
activator from the aggressive environment of the organism, at the same time
preserving the ability of the enzyme to interact with certain external agents
through a system of pores.
In particular, the matrix lets in
plasminogen, a proenzyme naturally occurring in blood plasma. When plasminogen
meets plasminogen activator inside the matrix, clot-dissolving plasmin forms.
Yulia Chapurina, laboratory
researcher and first author of the paper, set up several in vitro experiments
that helped demonstrate just how effective the film is.
“In order to test how our improved
vascular graft worked, we grew an artificial clot made of blood plasma mixed
with thrombin and placed it inside the graft,” said Chapurina.
“The results of the experiment
amazed us. Very soon the clot started to dissolve and leak through the graft.
In reality, our coating would destroy clots at the stage of formation,
constantly ensuring an unobstructed blood flow in the graft,” said Chapurina.
Latest-generation vascular grafts
rely on drug-eluting technology, that is, they actively release medicine into
the blood.
The lifetime of such grafts is often
determined by the amount of drug stored within the graft, which, in essence,
means that they merely postpone the formation of clots.
The study was published in the
Journal of Medicinal Chemistry. — PTI
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